Associate Scientist I/II -- Protein Production

Associate Scientist I/II, Protein Production

Dragonfly Therapeutics is seeking a highly motivated scientist with a strong focus in Molecular Biology and Protein expression and purification to join the Biologics team. The successful candidate will play a pivotal role in the DNA cloning and rapid generation of antibodies and multi-specifics at various scales to streamline discovery efforts. The candidate would be responsible for optimization, and expansion of DNA cloning, protein expression and protein purification platforms in coordination with downstream functional teams. We offer a unique opportunity to learn, grow professionally and be part of a fun, dedicated and goal-oriented team.

Responsibilities:

• Manual and automated mammalian protein production (upstream and downstream)
• Bench-scale mammalian protein purification using column chromatography (Affinity, IEX, SEC, HIC) and FPLC/liquid chromatography (AKTA Pure, AKTA Avant)
• Molecular biology techniques including cloning, PCR, and plasmid DNA prep
• Mammalian cell culture (Expi293, ExpiCHO) utilizing strong aseptic technique
• Tecan liquid handler method development, optimization, validation, and execution for automated high-throughput (HTP) protein production (DNA cloning, mammalian protein expression (Expi293, ExpiCHO), and mammalian protein purification)
• Design of Experiments (DOE) applied statistical approach to upstream and downstream process development activities to expand existing HTP protein production capabilities and related QC activities
• Perform assorted analytical biochemistry techniques (Octet titer, SDS-PAGE, SEC-HPLC, protein quantification, LAL endotoxin, etc)
• Work closely with Biologics and Biology leadership teams to ensure that program timelines and milestones are met for the production processes
• Document observations and data in electronic lab
• Analyze and present data at group meetings

Qualifications:

• S. in relevant field with 2+ years of industry experience or M.S. with 0-1+ years of industry experience
• Familiarity with liquid handler platforms (Opentrons, Tecan) is preferred
• Experience with mammalian cell culture, molecular biology, protein purification, and analytical biochemistry techniques
• Experience in method development, optimization, and troubleshooting
• Capable of operating independently with high level critical thinking and problem-solving experience
• Must be detail-oriented with excellent organizational skills; diligent documentation and highly efficient time management are required
• Previous success operating in a fast-paced, dynamic work environment
• Excellent communication and interpersonal skills