Flow Cytometry Contractor

Position Overview:

We are currently seeking a motivated, independent, and experienced research associate or associate scientist to join and contribute to a multidisciplinary program team focused on developing novel engineered prime edited ex vivo cell and in vivo therapies. The candidate will collaborate with scientists across multiple project teams to provide critical, on-site, scientific and operational flow cytometry support.

Primary responsibilities:

• Perform flow cytometry-based multi-parameter cell sorting and analysis including instrument set-up, compensation control preparation, PMT voltage adjustments, experimental settings selection, sample collection (sorting), troubleshooting, and cleaning.
• Participate in upstream sample processing for flow cytometry analysis and sorting, including tissue dissociation and antibody staining of cells.
• Perform maintenance on and schedule service for all flow cytometry analyzers and sorters.
• Serve as a resource for users; help scientists solve problems with their instrument operations and improve their skills in all aspects of FACS instrument function.
• Participate in generating, validating, and updating standard operating procedures for use of all flow cytometry instrumentation.
• Become familiar with relevant software used in the facility and assist facility users in learning to use this software.
• Respond to queries, communications, and workflows to ensure consistency and timely resolution.
• Manage inventory for materials, reagents, and consumables for flow cytometry core facility.
• Maintain detailed documentation of experiments in ELN system.

Qualifications:

• BS 6+, MS 5+, PhD 1-4+ years of relevant research experience
• 3+ years of experience with one or more of the following instrumentation systems: BD Aria/Fusion, BD Symphony, LSR, or Fortessa, Sony MA900 or SH800, Agilent Quanteon, Thermo Fisher Attune; Flow cytometry core experience preferred.
• Sufficiently high knowledge of flow cytometry and sorting theory and applications to train new users and assist in troubleshooting.
• Expertise in designing complex flow cytometry antibody panels with appropriate standards and controls, performing complex sorts on rare cell populations, analyzing and interpreting complex data sets
• Experience with biological cell sample processing relevant to flow cytometry, including working with whole blood, cell lines, and dissociated tissues.
• Familiar with techniques and instruments for mechanical and enzymatic tissue dissociation.
• Experience collaborating on cross-functional teams, willingness to roll-up-sleeves to get things done.
• Must be able to work in a laboratory, controlled environments requiring personal protective equipment (e.g., lab coat, safety glasses, etc.) in laboratory. 
• Job pace may be fast and job completion demands may be high. 
• Must be able to remain in a stationary position more than 25% of the time.